No trains will run from Stirling or Falkirk to Edinburgh later this month.
Network Rail say they are carrying out "urgent" work to prevent rockfall on the route.
It means disruption from 29th January to 2nd February.
ScotRail are asking people to only travel if essential.
Glasgow Queen Street to Edinburgh via Falkirk High services will terminate at Linlithgow.
Dunblane to Edinburgh services will terminate at Falkirk Grahamston.
Phil Campbell, ScotRail customer operations director, said: “Due to these engineering works, there will be significant disruption to the services ScotRail can operate between Edinburgh and Glasgow, Bathgate, Linlithgow, Stirling, and Inverness.
“We understand the disruption this will cause to customers' journeys, but it is essential that Network Rail carries out these works.
“ScotRail’s advice to customers travelling between Edinburgh and Glasgow via Falkirk High or via Bathgate, and between Edinburgh and Dunblane, is that you should only travel if your journey is essential.
“Customers travelling between Inverness and Edinburgh should also be aware that journey times may be longer as those services are diverted via Fife.
“We are working hard to finalise plans to provide alternative travel and will communicate that to customers in the coming days.”
Report into Falkirk Council finances
Rise in spiking incidents
Concern over Forth Valley road casualties
Falkirk and Grangemouth Growth Deal signed