No trains will run across the Forth Valley today, as workers stage industrial action.
Members of the RMT union are walking out in a dispute over pay.
The trade union's rejected an offer from Scotrail.
More than 2,000 members of staff are to strike today.
It means no services at all will run to Stirling, Falkirk or Alloa.
Disruption is expected for people heading to Murrayfield, for Scotland's match against Australia.
David Simpson, ScotRail Service Delivery Director, said:
“We’re really disappointed that the dispute with the RMT remains ongoing, given the strength of the improved pay offer ScotRail made.
“Further strike action has a damaging effect on railway’s recovery at a time when we should be encouraging more people to travel. Instead, our customers are being severely impacted and our staff losing out through lost wages.
“Regrettably, we won’t be able to operate the vast majority of our services on Saturday, 29 October, and customers should expect significant disruption to services due to the large proportion of ScotRail staff who are members of the RMT trade union.
“We’re advising customers, including rugby fans intending to travelling to Scotland’s first autumn international at Murrayfield, to seek alternative means of transport and to only travel by train if they really need to.
“We remain open to resolving this dispute.”